Improving the quality of quantitative polymerase chain reaction experiments: 15 years of MIQE

Authors: Bustin, Stephen A.

Source: In Molecular Aspects of Medicine April 2024 96

RT-qPCR Testing and Performance Metrics in the COVID-19 Era.

Authors: Bustin, Stephen A.¹ (AUTHOR) stephen.bustin@aru.ac.uk

Source: International Journal of Molecular Sciences. Sep2024, Vol. 25 Issue 17, p9326. 17p.

Subject Terms: *MEDICAL personnel, *COVID-19 pandemic, *COMMUNICABLE diseases, *DISEASE outbreaks, *POLYMERASE chain reaction

Disclosing quantitative RT‐PCR raw data during manuscript submission: a call for action

Authors: Untergasser, Andreas, Hellemans, Jan, Pfaffl, Michael W., Ruijter, Jan M., Hoff, Maurice J. B., Dragomir, Mihnea P., Adamoski, Douglas, Dias, Sandra Martha Gomes, Reis, Rui Manuel, Ferracin, Manuela, Dias‐Neto, Emmanuel, Marsh, Ian, Kubista, Mikael, Fabbri, Muller, Goel, Ajay, Slabý, Ondřej, Knutsen, Erik, Chen, Baoqing, Negrini, Massimo, Mimori, Koshi, Pichler, Martin, Papatriantafyllou, Maria, Anfossi, Simone, Schmittgen, Thomas D., Huggett, Jim, Bustin, Stephen, Vandesompele, Jo, Calin, George A.

Source: Molecular oncology. 17(5):713-717

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100182069733.0x000001&indx=1&recIds=ETOCvdc_100182069733.0x000001

FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.

Authors: Bustin, Stephen A.¹ (AUTHOR) sara.kirvell1@aru.ac.uk, Kirvell, Sara¹ (AUTHOR) tanianolan@btinternet.com, Nolan, Tania¹ (AUTHOR), Shipley, Gregory L.² (AUTHOR) gshipley14@me.com

Source: International Journal of Molecular Sciences. Mar2024, Vol. 25 Issue 5, p2773. 19p.

Subject Terms: *POLYMERASE chain reaction, *MOLECULAR diagnosis

Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics

Authors: Whale, Alexandra S., von der Heide, Eva K., Kohlenberg, Max, Brinckmann, Anja, Baedker, Silke, Karalay, Oezlem, Fernandez-Gonzalez, Ana, Busby, Eloise J., Bustin, Stephen A., Hauser, Heiko, Missel, Andreas, O'Sullivan, Denise M., Huggett, Jim F., Pfaffl, Michael W., Nolan, Tania

Source: Methods. 201:5-14

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100161073487.0x000001&indx=1&recIds=ETOCvdc_100161073487.0x000001

Development of a phenotypic screening assay to measure activation of cancer-associated fibroblasts.

Authors: Ilg, Marcus M., Lapthorn, Alice R., Harding, Sophie L., Minhas, Tariq, Koduri, Gouri, Bustin, Stephen A., Cellek, Selim

Source: Frontiers in Pharmacology; 2025, p1-13, 13p

Subject Terms: EXTRACELLULAR matrix, CELL physiology, DRUG discovery, CANCER cells, PERIOSTIN

Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance

Authors: Bivins, Aaron, Kaya, Devrim, Bibby, Kyle, Simpson, Stuart L., Bustin, Stephen A., Shanks, Orin C., Ahmed, Warish

Source: In Water Research 15 September 2021 203

Advances in Molecular Medicine: Unravelling Disease Complexity and Pioneering Precision Healthcare.

Authors: Bustin, Stephen A.¹ (AUTHOR) stephen.bustin@aru.ac.uk, Jellinger, Kurt A.² (AUTHOR) stephen.bustin@aru.ac.uk

Source: International Journal of Molecular Sciences. Sep2023, Vol. 24 Issue 18, p14168. 8p.

Subject Terms: *MOLECULAR pathology, *MEDICAL care, *MEDICAL personnel, *DRUGS, *THERAPEUTICS, *PRECISION farming, *TRANSCRANIAL direct current stimulation

Phosphodiesterase Type 5 Inhibitors and Selective Estrogen Receptor Modulators Can Prevent But Not Reverse Myofibroblast Transformation in Peyronie's Disease

Authors: Ilg, Marcus M., Stafford, Simon J., Mateus, Marta, Bustin, Stephen A., Carpenter, Michael J., Muneer, Asif, Bivalacqua, Trinity J., Ralph, David J., Cellek, Selim

Source: In The Journal of Sexual Medicine October 2020 17(10):1848-1864

Parameters for Successful PCR Primer Design

Authors: Bustin, Stephen A., Mueller, Reinhold, Nolan, Tania

Source: Methods in molecular biology. 2065:5-22

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCRN621892907&indx=1&recIds=ETOCRN621892907

Maximising the Use of Scarce qPCR Master Mixes.

Authors: Bustin, Stephen¹ (AUTHOR) sara.kirvell1@aru.ac.uk, Bustin, Claire² (AUTHOR) c.bustin1@nhs.net, Kirvell, Sara¹ (AUTHOR) tanianolan@btinternet.com, Nolan, Tania¹ (AUTHOR), Mueller, Reinhold³ (AUTHOR) reinholdmueller7@gmail.com, Shipley, Gregory⁴ (AUTHOR) gshipley14@me.com

Source: International Journal of Molecular Sciences. Aug2022, Vol. 23 Issue 15, p8486-8486. 14p.

Subject Terms: *COLLEGE laboratories, *PRICES, *COVID-19 pandemic, *DIAGNOSIS methods, *SCARCITY

qPCR primer design revisited

Authors: Bustin, Stephen, Huggett, Jim

Source: In Biomolecular Detection and Quantification December 2017 14:19-28

Molecular Pathology, Diagnostics and Therapeutics: A Story of Success in 2022.

Authors: Bustin, Stephen A.¹ (AUTHOR) stephen.bustin@gmail.com

Source: International Journal of Molecular Sciences. Mar2023, Vol. 24 Issue 5, p5063. 3p.

Subject Terms: *MOLECULAR pathology, *THERAPEUTICS, *MOLECULAR biology, *ADVISORY boards, *DRUG design

Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B

Authors: Dhillon, Harvinder S., Johnson, Gemma, Shannon, Mark, Greenwood, Christina, Roberts, Doug, Bustin, Stephen

Source: In Biomolecular Detection and Quantification December 2016 10:2-8

Proximity assays for sensitive quantification of proteins

Authors: Greenwood, Christina, Ruff, David, Kirvell, Sara, Johnson, Gemma, Dhillon, Harvinder S., Bustin, Stephen A.

Source: In Biomolecular Detection and Quantification June 2015 4:10-16

The reproducibility of biomedical research: Sleepers awake!

Authors: Bustin, Stephen A.

Source: In Biomolecular Detection and Quantification December 2014 2:35-42

Influence of trypsinization and alternative procedures for cell preparation before RNA extraction on RNA integrity

Authors: Vrtačnik, Peter, Kos, Špela, Bustin, Stephen A., Marc, Janja, Ostanek, Barbara

Source: In Analytical Biochemistry 15 October 2014 463:38-44

CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19.

Authors: Bustin, Stephen¹ Stephen.bustin@aru.ac.uk, Coward, Amy², Sadler, Garry², Teare, Louise², Nolan, Tania¹

Source: Scientific Reports. 12/17/2020, Vol. 10 Issue 1, p1-13. 13p.

Subject Terms: *COVID-19, *SARS-CoV-2, *VIRAL disease diagnosis, *POLYMERASE chain reaction, *GENETIC mutation, *GENOTYPES

Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh

Authors: Ansari, Wahedul Karim, Parvej, Md. Shafiullah, El Zowalaty, Mohamed E., Jackson, Sally, Bustin, Stephen A., Ibrahim, Adel K., El Zowalaty, Ahmed E., Rahman, Md. Tanvir, Zhang, Han, Khan, Mohammad Ferdousur Rahman, Ahamed, Md. Mostakin, Rahman, Md. Fasiur, Rahman, Marzia, Nazir, K.H.M. Nazmul Hussain, Ahmed, Sultan, Hossen, Md. Liakot, Kafi, Md. Abdul, Yamage, Mat, Debnath, Nitish C., Ahmed, Graba, Ashour, Hossam M., Masudur Rahman, Md., Noreddin, Ayman, Rahman, Md. Bahanur

Source: Veterinary microbiology. 193:49-59

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100060629234.0x000001&indx=1&recIds=ETOCvdc_100060629234.0x000001

RNA biomarkers in colorectal cancer

Authors: Bustin, Stephen A., Murphy, Jamie

Source: In Methods January 2013 59(1):116-125