High-throughput platform for label-free sorting of 3D spheroids using deep learning

Authors: Claudia Sampaio da Silva, Julia Alicia Boos, Jonas Goldowsky, Manon Blache, Noa Schmid, Tim Heinemann, Christoph Netsch, Francesca Luongo, Stéphanie Boder-Pasche, Gilles Weder, Alba Pueyo Moliner, Roos-Anne Samsom, Ary Marsee, Kerstin Schneeberger, Ali Mirsaidi, Bart Spee, Thomas Valentin, Andreas Hierlemann, Vincent Revol

Source: Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)

Subject Terms: automation, high-throughput sorting, multi-cellular spheroids, 3D bioprinting, machine learning, transfer learning, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fbioe.2024.1432737/full; https://doaj.org/toc/2296-4185

Access URL: https://doaj.org/article/22da439e01c74baf80d340ea0048f0d2

Treatment of Canine Type 1 Diabetes Mellitus: The Long Road from Twice Daily Insulin Injection towards Long-Lasting Cell-Based Therapy

Authors: Flavia C. M. Oliveira, Annemarie W. Y. Voorbij, Elisa C. Pereira, Leonor M. M. Alves e Almeida, Geanne R. Moraes, Joana T. De Oliveira, Boyd H. T. Gouw, Sabrina A. M. Legatti, Hans S. Kooistra, Bart Spee, Andre M. C. Meneses, Louis C. Penning

Source: Organoids, Vol 3, Iss 2, Pp 67-82 (2024)

Subject Terms: dogs, insulin, pancreas, organoids, regenerative medicine, Cytology, QH573-671

File Description: electronic resource

Relation: https://www.mdpi.com/2674-1172/3/2/6; https://doaj.org/toc/2674-1172

Access URL: https://doaj.org/article/c769440975544fbebe1a015a09f86e68

Cholangiocyte organoids to study drug-induced injury

Authors: Zhenguo Wang, Chen Xing, Luc J. W. van der Laan, Monique M. A. Verstegen, Bart Spee, Rosalinde Masereeuw

Source: Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-15 (2024)

Subject Terms: Intrahepatic cholangiocyte organoids, Cholangiocytes, Drug induced bile duct injury, Advanced in vitro model, Chlorpromazine, Bile acid, Medicine (General), R5-920, Biochemistry, QD415-436

File Description: electronic resource

Relation: https://doaj.org/toc/1757-6512

Access URL: https://doaj.org/article/8d28353034a843c4be8fec25debc89e5

Intestinal Cells-on-Chip for Permeability Studies

Authors: Marit Keuper-Navis, Hossein Eslami Amirabadi, Joanne Donkers, Markus Walles, Birk Poller, Bo Heming, Lisanne Pieters, Bjorn de Wagenaar, Adam Myszczyszyn, Theo Sinnige, Bart Spee, Rosalinde Masereeuw, Evita van de Steeg

Source: Micromachines, Vol 15, Iss 12, p 1464 (2024)

Subject Terms: intestine-on-chip, cell monolayer, in vitro model, intestinal absorption, intestinal barrier, permeability, Mechanical engineering and machinery, TJ1-1570

File Description: electronic resource

Relation: https://www.mdpi.com/2072-666X/15/12/1464; https://doaj.org/toc/2072-666X

Access URL: https://doaj.org/article/f41a5b02059f4c3296e4cc4e52b1e8d6

Locational memory of macrovessel vascular cells is transcriptionally imprinted

Authors: Talitha C. F. Spanjersberg, Loes A. Oosterhoff, Hedwig S. Kruitwagen, Noortje A. M. van den Dungen, Johannes C. M. Vernooij, Folkert W. Asselbergs, Michal Mokry, Bart Spee, Magdalena Harakalova, Frank G. van Steenbeek

Source: Scientific Reports, Vol 13, Iss 1, Pp 1-14 (2023)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/084d9af137ee4c1b8e300e79be3a0038

The application of organ-on-chip models for the prediction of human pharmacokinetic profiles during drug development

Authors: Marit Keuper-Navis, Markus Walles, Birk Poller, Adam Myszczyszyn, Thomas K. van der Made, Joanne Donkers, Hossein Eslami Amirabadi, Martijn J. Wilmer, Saskia Aan, Bart Spee, Rosalinde Masereeuw, Evita van de Steeg

Source: Pharmacological Research, Vol 195, Iss , Pp 106853- (2023)

Subject Terms: Organ-on-chip, Drug development, Pharmacokinetics, ADME, Therapeutics. Pharmacology, RM1-950

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S1043661823002098; https://doaj.org/toc/1096-1186

Access URL: https://doaj.org/article/06d95e9d904945618f9e6018cbd79e94

Are slaughterhouse-obtained livers suitable for use in perfusion research?

Authors: Alicia Ruppelt, Iris Pijnenburg, Claire Pappers, Roos-Anne Samsom, Linda Kock, Guy C. M. Grinwis, Bart Spee, Marco Rasponi, Marco Stijnen

Source: Journal of International Medical Research, Vol 51 (2023)

Subject Terms: Medicine (General), R5-920

File Description: electronic resource

Relation: https://doaj.org/toc/1473-2300

Access URL: https://doaj.org/article/71a0c16bc1df4bd097e5bca873720c20

A human kidney and liver organoid‐based multi‐organ‐on‐a‐chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell‐derived extracellular vesicles

Authors: Vivian V. T. Nguyen, Shicheng Ye, Vasiliki Gkouzioti, Monique E. vanWolferen, Fjodor Yousef Yengej, Dennis Melkert, Sofia Siti, Bart deJong, Paul J. Besseling, Bart Spee, Luc J. W. van derLaan, Reyk Horland, Marianne C. Verhaar, Bas W. M. vanBalkom

Source: Journal of Extracellular Vesicles, Vol 11, Iss 11, Pp n/a-n/a (2022)

Subject Terms: 3Rs, EV‐based therapeutics, micro‐physiological models, renal injury, Cytology, QH573-671

File Description: electronic resource

Relation: https://doaj.org/toc/2001-3078

Access URL: https://doaj.org/article/0bfe9b51c350456fa7665cedf2f8b71f

Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease

Authors: Monique M. A. Verstegen, Floris J. M. Roos, Ksenia Burka, Helmuth Gehart, Myrthe Jager, Maaike de Wolf, Marcel J. C. Bijvelds, Hugo R. de Jonge, Arif I. Ardisasmita, Nick A. van Huizen, Henk P. Roest, Jeroen de Jonge, Michael Koch, Francesco Pampaloni, Sabine A. Fuchs, Imre F. Schene, Theo M. Luider, Hubert P. J. van der Doef, Frank A. J. A. Bodewes, Ruben H. J. de Kleine, Bart Spee, Gert-Jan Kremers, Hans Clevers, Jan N. M. IJzermans, Edwin Cuppen, Luc J. W. van der Laan

Source: Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)

Subject Terms: Medicine, Science

File Description: electronic resource

Relation: https://doaj.org/toc/2045-2322

Access URL: https://doaj.org/article/eb9e7d98f7234f40b94f21db6d99f8db

Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection

Authors: Yunlong Li, Changbo Qu, Bart Spee, Ruyi Zhang, Louis C. Penning, Robert A. de Man, Maikel P. Peppelenbosch, Hille Fieten, Qiuwei Pan

Source: Irish Veterinary Journal, Vol 73, Iss 1, Pp 1-5 (2020)

Subject Terms: HEV, Dog, Cat, Horse, Seroprevalence, Infection, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: http://link.springer.com/article/10.1186/s13620-020-00158-y; https://doaj.org/toc/2046-0481

Access URL: https://doaj.org/article/e5fda4415dfe41d5bd13d11750f5b386

Human Cholangiocytes Form a Polarized and Functional Bile Duct on Hollow Fiber Membranes

Authors: Zhenguo Wang, João Faria, Luc J. W. van der Laan, Louis C. Penning, Rosalinde Masereeuw, Bart Spee

Source: Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)

Subject Terms: intrahepatic cholangiocyte organoids, cholangiocytes, bioengineered bile duct, hollow fiber membrane, monolayer, polarity, Biotechnology, TP248.13-248.65

File Description: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fbioe.2022.868857/full; https://doaj.org/toc/2296-4185

Access URL: https://doaj.org/article/cab7f83ee5244511aa9e4af4fb961bd8

Drug Metabolism of Hepatocyte-like Organoids and Their Applicability in In Vitro Toxicity Testing

Authors: Manon C. Bouwmeester, Yu Tao, Susana Proença, Frank G. van Steenbeek, Roos-Anne Samsom, Sandra M. Nijmeijer, Theo Sinnige, Luc J. W. van der Laan, Juliette Legler, Kerstin Schneeberger, Nynke I. Kramer, Bart Spee

Source: Molecules, Vol 28, Iss 2, p 621 (2023)

Subject Terms: drug-induced liver injury, hepatic in vitro model, hepatotoxicity, intrahepatic cholangiocyte organoids, hepatocyte-like cells, Organic chemistry, QD241-441

File Description: electronic resource

Relation: https://www.mdpi.com/1420-3049/28/2/621; https://doaj.org/toc/1420-3049

Access URL: https://doaj.org/article/8b733aeea46746d7985ffe97537d2bfb

Identification of potential drugs for treatment of hepatic lipidosis in cats using an in vitro feline liver organoid system

Authors: Maya W. Haaker, Hedwig S. Kruitwagen, Arie B. Vaandrager, Martin Houweling, Louis C. Penning, Martijn R. Molenaar, Monique E. vanWolferen, Loes A. Oosterhoff, Bart Spee, J. Bernd Helms

Source: Journal of Veterinary Internal Medicine, Vol 34, Iss 1, Pp 132-138 (2020)

Subject Terms: AICAR, diacylglycerol O‐acyltransferase 1 inhibitor, hepatic organoids, perilipin 2, steatosis, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676

Access URL: https://doaj.org/article/9e1ab320d6b74ca9bb639a32d61656eb

Efficacy of orally administered sodium benzoate and sodium phenylbutyrate in dogs with congenital portosystemic shunts

Authors: Giora van Straten, Diewke vanDalen, Sietske J. Mesu, Jan Rothuizen, Erik Teske, Bart Spee, Robert P. Favier, Ingeborg M. vanGeijlswijk

Source: Journal of Veterinary Internal Medicine, Vol 33, Iss 3, Pp 1331-1335 (2019)

Subject Terms: canine, hepatic encephalopathy, hyperammonemia, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676

Access URL: https://doaj.org/article/4bf6544365344e0ebaec58608486d724

Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling

Authors: Melanie Krüger, Alicia Ruppelt, Benjamin Kappler, Elke Van Soest, Roos Anne Samsom, Guy C. M. Grinwis, Niels Geijsen, J. Bernd Helms, Marco Stijnen, Linda M. Kock, Marco Rasponi, Hans S. Kooistra, Bart Spee

Source: Bioengineering, Vol 9, Iss 9, p 471 (2022)

Subject Terms: hepatic diseases, machine perfusion, acetaminophen, steatosis, Technology, Biology (General), QH301-705.5

File Description: electronic resource

Relation: https://www.mdpi.com/2306-5354/9/9/471; https://doaj.org/toc/2306-5354

Access URL: https://doaj.org/article/6cef8376ae704fadbfe270662ee227a7

Association of circulating microRNA‐122 and microRNA‐29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers

Authors: Manabu Sakai, Bart Spee, Guy C. M. Grinwis, Louis C. Penning, Monique E. vanWolferen, Luc J. W. van derLaan, Hille Fieten

Source: Journal of Veterinary Internal Medicine, Vol 33, Iss 1, Pp 151-157 (2019)

Subject Terms: canine, cirrhosis, dog, fibrosis, liver injury, Veterinary medicine, SF600-1100

File Description: electronic resource

Relation: https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676

Access URL: https://doaj.org/article/b3d818b5ca1a44fabe4546525ba47d27

Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis

Authors: Hedwig S. Kruitwagen, Loes A. Oosterhoff, Ingrid G.W.H. Vernooij, Ingrid M. Schrall, Monique E. van Wolferen, Farah Bannink, Camille Roesch, Lisa van Uden, Martijn R. Molenaar, J. Bernd Helms, Guy C.M. Grinwis, Monique M.A. Verstegen, Luc J.W. van der Laan, Meritxell Huch, Niels Geijsen, Robert G. Vries, Hans Clevers, Jan Rothuizen, Baukje A. Schotanus, Louis C. Penning, Bart Spee

Source: Stem Cell Reports, Vol 8, Iss 4, Pp 822-830 (2017)

Subject Terms: Medicine (General), R5-920, Biology (General), QH301-705.5

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2213671117300826; https://doaj.org/toc/2213-6711

Access URL: https://doaj.org/article/1b75311bbf3843328b88a26d9590e37a

ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells[S]

Authors: Maidina Tuohetahuntila, Martijn R. Molenaar, Bart Spee, Jos F. Brouwers, Martin Houweling, Arie B. Vaandrager, J. Bernd Helms

Source: Journal of Lipid Research, Vol 57, Iss 7, Pp 1162-1174 (2016)

Subject Terms: vitamin A, lipase, lipolysis and fatty acid metabolism, lipid droplets, lipidomics, heavy isotope labeling, Biochemistry, QD415-436

File Description: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S0022227520351543; https://doaj.org/toc/0022-2275

Access URL: https://doaj.org/article/e9fd671f5e4a44a8a318a75e8e2a5506

Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels

Authors: Loes A. Oosterhoff, Hedwig S. Kruitwagen, Monique E. van Wolferen, Bas W.M. van Balkom, Michal Mokry, Nico Lansu, Noortje A.M. van den Dungen, Louis C. Penning, Talitha C.F. Spanjersberg, Johannes W. de Graaf, Tomas Veenendaal, Flin Zomerdijk, Joost O. Fledderus, Bart Spee, Frank G. van Steenbeek

Source: Frontiers in Physiology, Vol 10 (2019)

Subject Terms: angiogenesis, cell model system, endothelial cells, vascular cell interaction, vascular smooth muscle cells, Physiology, QP1-981

File Description: electronic resource

Relation: https://www.frontiersin.org/article/10.3389/fphys.2019.00101/full; https://doaj.org/toc/1664-042X

Access URL: https://doaj.org/article/d3bcd76059fd4137b465861aad4ad624

The Menkes and Wilson disease genes counteract in copper toxicosis in Labrador retrievers: a new canine model for copper-metabolism disorders

Authors: Hille Fieten, Yadvinder Gill, Alan J. Martin, Mafalda Concilli, Karen Dirksen, Frank G. van Steenbeek, Bart Spee, Ted S. G. A. M. van den Ingh, Ellen C. C. P. Martens, Paola Festa, Giancarlo Chesi, Bart van de Sluis, Roderick H. J. H. Houwen, Adrian L. Watson, Yurii S. Aulchenko, Victoria L. Hodgkinson, Sha Zhu, Michael J. Petris, Roman S. Polishchuk, Peter A. J. Leegwater, Jan Rothuizen

Source: Disease Models & Mechanisms, Vol 9, Iss 1, Pp 25-38 (2016)

Subject Terms: ATP7A, ATP7B, Dog, Liver, Medicine, Pathology, RB1-214

File Description: electronic resource

Relation: http://dmm.biologists.org/content/9/1/25; https://doaj.org/toc/1754-8411; https://doaj.org/toc/1754-8403

Access URL: https://doaj.org/article/ed1cab5637da4a468e9d141775e641c2