Analysis of ribonucleotide content in the genomic DNA of ribonuclease H2 A subunit (RH2A)-knockout NIH3T3 cells after transient expression of wild-type RH2A or RH2A variants with an Aicardi–Goutières syndrome-causing mutation

Authors: Kandabashi, Mako, Yano, Haruna, Hara, Haruka, Ogawa, Saori, Kamoda, Kana, Ishibashi, Shu, Himeda, Kohei, Baba, Misato, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Journal of biochemistry. 172(4):225-231

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100162532347.0x000001&indx=1&recIds=ETOCvdc_100162532347.0x000001

Accurate fidelity analysis of the reverse transcriptase by a modified next-generation sequencing

Authors: Okano, Hiroyuki, Baba, Misato, Hidese, Ryota, Iida, Kei, Li, Tongyang, Kojima, Kenji, Takita, Teisuke, Yanagihara, Itaru, Fujiwara, Shinsuke, Yasukawa, Kiyoshi

Source: In Enzyme and Microbial Technology August 2018 115:81-85

High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies

Authors: Okano, Hiroyuki, Baba, Misato, Kawato, Katsuhiro, Hidese, Ryota, Yanagihara, Itaru, Kojima, Kenji, Takita, Teisuke, Fujiwara, Shinsuke, Yasukawa, Kiyoshi

Source: In Journal of Bioscience and Bioengineering March 2018 125(3):275-281

Construction and characterization of ribonuclease H2 knockout NIH3T3 cells

Authors: Tsukiashi, Motoki, Baba, Misato, Kojima, Kenji, Himeda, Kohei, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Journal of biochemistry. 165(3):249-256

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100078425403.0x000001&indx=1&recIds=ETOCvdc_100078425403.0x000001

Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase

Authors: Okano, Hiroyuki, Katano, Yuta, Baba, Misato, Fujiwara, Ayako, Hidese, Ryota, Fujiwara, Shinsuke, Yanagihara, Itaru, Hayashi, Tsukasa, Kojima, Kenji, Takita, Teisuke, Yasukawa, Kiyoshi

Source: In Enzyme and Microbial Technology January 2017 96:111-120

Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region

Authors: Narukawa, Yutaro, Kandabashi, Mako, Li, Tongyang, Baba, Misato, Hara, Haruka, Kojima, Kenji, Iida, Kei, Hiyama, Takayoshi, Yokoe, Sho, Yamazaki, Tomomi, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Protein engineering, design & selection. 34

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100168253328.0x000001&indx=1&recIds=ETOCvdc_100168253328.0x000001

Next-generation sequencing-based analysis of reverse transcriptase fidelity

Authors: Yasukawa, Kiyoshi, Iida, Kei, Okano, Hiroyuki, Hidese, Ryota, Baba, Misato, Yanagihara, Itaru, Kojima, Kenji, Takita, Teisuke, Fujiwara, Shinsuke

Source: Biochemical and biophysical research communications. 492(2):147-153

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100063603685.0x000001&indx=1&recIds=ETOCvdc_100063603685.0x000001

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system

Authors: Katano, Yuta, Li, Tongyang, Baba, Misato, Nakamura, Miyo, Ito, Masaaki, Kojima, Kenji, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Bioscience, biotechnology, and biochemistry. 81(12):2339-2345

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100053463530.0x000001&indx=1&recIds=ETOCvdc_100053463530.0x000001

High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase

Authors: Okano, Hiroyuki, Baba, Misato, Yamasaki, Tomomi, Hidese, Ryota, Fujiwara, Shinsuke, Yanagihara, Itaru, Ujiiye, Takeshi, Hayashi, Tsukasa, Kojima, Kenji, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Biochemical and biophysical research communications. 487(1):128-133

Availability: http://explore.bl.uk/primo_library/libweb/action/display.do?tabs=detailsTab&gathStatTab=true&ct=display&fn=search&doc=ETOCvdc_100047249273.0x000001&indx=1&recIds=ETOCvdc_100047249273.0x000001

Val143 of human ribonuclease H2 is not critical for, but plays a role in determining catalytic activity and substrate specificity.

Authors: Baba, Misato¹ (AUTHOR), Kojima, Kenji¹ (AUTHOR), Nishimura, Takuto¹ (AUTHOR), Sugiura, Takuya¹ (AUTHOR), Takita, Teisuke¹ (AUTHOR), Uehara, Ryo^2,3 (AUTHOR), Crouch, Robert J.² (AUTHOR), Yasukawa, Kiyoshi¹ (AUTHOR) yasukawa@kais.kyoto-u.ac.jp

Source: PLoS ONE. 2/18/2020, Vol. 15 Issue 2, p1-16. 16p.

Subject Terms: *CATALYTIC activity, *RIBONUCLEOSIDE diphosphate reductase, *STERIC hindrance, *RIBONUCLEASES, *AMINO acids, *TRYPTOPHAN

Characterization of six recombinant human RNase H2 bearing Aicardi-Goutiéres syndrome causing mutations.

Authors: Nishimura, Takuto¹ (AUTHOR), Baba, Misato¹ (AUTHOR), Ogawa, Saori¹ (AUTHOR), Kojima, Kenji¹ (AUTHOR), Takita, Teisuke¹ (AUTHOR), Crouch, Robert J² (AUTHOR), Yasukawa, Kiyoshi¹ (AUTHOR) yasukawa@kais.kyotou.ac.jp

Source: Journal of Biochemistry. Dec2019, Vol. 166 Issue 6, p537-545. 9p.

Subject Terms: *GEL permeation chromatography, *CIRCULAR dichroism, *RIBONUCLEOSIDE diphosphate reductase

RNA/DNA structures recognized by RNase H2.

Authors: Kojima, Kenji¹ (AUTHOR), Baba, Misato¹ (AUTHOR), Tsukiashi, Motoki¹ (AUTHOR), Nishimura, Takuto¹ (AUTHOR), Yasukawa, Kiyoshi¹ (AUTHOR) yasukawa@kais.kyoto-u.ac.jp

Source: Briefings in Functional Genomics. May2019, Vol. 18 Issue 3, p169-173. 5p.

Subject Terms: *RIBONUCLEASE H, *RIBONUCLEOSIDE diphosphate reductase, *DOUBLE-strand DNA breaks, *RNA, *DNA repair, *DNA structure, *SINGLE-stranded DNA

Construction and characterization of ribonuclease H2 C subunit-knockout NIH3T3 cells

Authors: Hara, Haruka, Yano, Haruna, Akazawa, Kaho, Kamoda, Kana, Kandabashi, Mako, Baba, Misato, Takita, Teisuke, Yasukawa, Kiyoshi

Source: Bioscience, Biotechnology, and Biochemistry; August 2023, Vol. 87 Issue: 8 p865-876, 12p

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